Overview: This lab exercise will most likely take 1-3 days. The lab performed will be forcing E.coli to take up and express an rfp protein through the process called transformation. Essentially the bacteria becomes a mutated form of E. coli. As we know from previous activities in the unit, mutation is a mechanism that drives evolution. Students will have pre-lab and post lab activities.
Objectives: Students will be able to: - Review notes on the process of transformation. - Follow directions from the teacher and the lab manual. - Follow safety instructions of the lab. - Perform the lab. - Answer post lab conclusion questions - Hypothesize/predict how they think this new mutated type of E. coli could evolve.
Opening: (Day 1) We will review the process of transformation by using the power point slides that accompany the Amgen Bruce Wallace kit. Students will receive a copy of the slides to follow along and will be able to ask any clarifying questions needed before the lab is performed.
Body: Students will spend the main portion of the class practicing effective pipette use and following the directions from “Lab 1: An Introduction to Microvolumentrics and Pipetting”. This will allow them to get used to the materials they will be using when doing the actual transformation lab. Closing: Students will conclude the class by answering the pre-lab questions for Lab 5 (found in the Teacher’s Guide of the Amgen Lab booklet pg 28).
Overview:
This lab exercise will most likely take 1-3 days. The lab performed will be forcing E.coli to take up and express an rfp protein through the process called transformation. Essentially the bacteria becomes a mutated form of E. coli. As we know from previous activities in the unit, mutation is a mechanism that drives evolution. Students will have pre-lab and post lab activities.
Objectives:
Students will be able to:
- Review notes on the process of transformation.
- Follow directions from the teacher and the lab manual.
- Follow safety instructions of the lab.
- Perform the lab.
- Answer post lab conclusion questions
- Hypothesize/predict how they think this new mutated type of E. coli could evolve.
Opening: (Day 1)
We will review the process of transformation by using the power point slides that accompany the Amgen Bruce Wallace kit. Students will receive a copy of the slides to follow along and will be able to ask any clarifying questions needed before the lab is performed.
Body:
Students will spend the main portion of the class practicing effective pipette use and following the directions from “Lab 1: An Introduction to Microvolumentrics and Pipetting”. This will allow them to get used to the materials they will be using when doing the actual transformation lab.
Closing:
Students will conclude the class by answering the pre-lab questions for Lab 5 (found in the Teacher’s Guide of the Amgen Lab booklet pg 28).
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